Diagnostic Testing · NPS Wellness & Aesthetics

Functional Diagnostic Testing

Advanced testing to uncover root causes — gut health, hormone balance, food sensitivities, and nutritional status — so we can build a truly personalized plan for you.

Going Beyond Standard Labs

Standard bloodwork has its place — but it often misses the deeper picture. At NPS Wellness, Stephanie Kertes, NP-C uses advanced functional diagnostic tests to look at how your gut is functioning, how your hormones are being produced and metabolized, how your immune system responds to foods, and whether your body has the nutrients it needs to thrive.

These tests are ordered as part of your Wellness Consult (Root-Cause) or other personalized health programs — not as isolated tests, but as tools that inform a comprehensive plan.

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GI-MAP

Gut microbiome & digestive health analysis

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DUTCH Test

Comprehensive hormone & metabolite analysis

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Food Sensitivity

96 or 184 IgG food sensitivity panels

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Hair Nutrient Analysis

Toxic & nutrient element screening from hair

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Saliva Hormones

LCMS hormone panel + 4-point cortisol via saliva

Gut Health

GI-MAP® Stool Test

The GI-MAP® (Gastrointestinal Microbial Assay Plus) from Diagnostic Solutions Laboratory is a comprehensive stool test that analyzes microbial DNA in the gastrointestinal tract using quantitative PCR (qPCR) technology. From a single stool sample, this advanced test identifies microorganisms and key markers related to digestion, immune activity, inflammation, and gut barrier health.

We also offer the GI-MAP + Zonulin upgrade, which adds an intestinal permeability marker — useful for evaluating what’s sometimes referred to as “leaky gut.”

Single stool sample collected at home
Quantitative PCR (qPCR) DNA technology
Results in approximately 10–12 business days
Results reviewed with Stephanie at follow-up

What the GI-MAP Evaluates

Pathogenic Bacteria

Campylobacter, Salmonella, Shigella, pathogenic E. coli strains, and C. difficile toxin genes — organisms that can contribute to GI infections or inflammation.

H. pylori & Virulence Factors

Detects H. pylori along with virulence genes (e.g. CagA) and antibiotic resistance markers — providing insight into treatment considerations.

Opportunistic Bacteria

Klebsiella, Pseudomonas, Streptococcus, and Staphylococcus — bacteria that may contribute to dysbiosis when overgrown.

Beneficial Bacteria

Bifidobacterium, Lactobacillus, Akkermansia muciniphila, and Faecalibacterium prausnitzii — key organisms supporting digestion and gut barrier health.

Parasites, Viruses & Fungi

Giardia, Cryptosporidium, Norovirus, Rotavirus, Adenovirus 40/41, and Candida species.

Digestive & Immune Markers

Pancreatic Elastase-1, Calprotectin, EPX/EDN (inflammation), Secretory IgA, and Anti-gliadin IgA.

+ Zonulin Add-On

Zonulin regulates tight junctions between intestinal cells. Elevated levels may indicate increased intestinal permeability — influencing immune activation and digestive symptoms.

Hormone Health

DUTCH® Hormone Testing

The DUTCH® Test (Dried Urine Test for Comprehensive Hormones) evaluates sex hormones, adrenal hormones, and their metabolites using dried urine samples collected throughout the day. Unlike a single-point blood draw, DUTCH testing reveals how hormones are produced, metabolized, and eliminated over a 24-hour period.

Stephanie uses DUTCH testing to evaluate hormonal balance, adrenal function, circadian rhythm patterns, and key metabolic markers — particularly for clients experiencing fatigue, mood changes, PMS, perimenopause or menopause symptoms, fertility concerns, sleep disruption, or unexplained weight changes.

Dried urine samples collected at home
Collected over a 24-hour period
Results in approximately 7–10 days
Results reviewed in detail with Stephanie

What DUTCH Testing Measures

Sex Hormones

Estradiol, Estrone, Estriol, Progesterone, Testosterone, and DHEA — evaluating production and balance in both men and women.

Hormone Metabolites

How estrogen, androgens, and cortisol are broken down — a key advantage DUTCH has over standard blood panels.

Adrenal & Stress Response

Cortisol, cortisone, and metabolites measured across the day to evaluate the diurnal pattern and adrenal function.

Organic Acid Markers

Select markers related to B-vitamin status, neurotransmitter metabolism, oxidative stress, and detoxification pathways.

Sleep & Oxidative Stress

Melatonin and 8-OHdG oxidative stress marker — evaluating sleep patterns and cellular stress levels.

Cortisol Awakening Response

Available on the DUTCH Plus panel — saliva samples capture the morning cortisol surge for deeper HPA axis assessment.

Available DUTCH Panels

DUTCH Complete

Sex hormones, cortisol rhythm, hormone metabolites, organic acids, melatonin & oxidative stress. Most commonly ordered.

DUTCH Plus

Everything in DUTCH Complete plus the Cortisol Awakening Response (CAR) via saliva — for deeper adrenal assessment.

DUTCH Cycle Mapping

For women with cycles — evaluates estrogen and progesterone patterns across the full month, including ovulation timing.

Immune Response Testing

IgG Food Sensitivity Panel

Food sensitivity testing from Alletess Medical Laboratory measures IgG antibody reactions to specific foods. Unlike a true food allergy (IgE-mediated), food sensitivities involve a delayed immune response — symptoms may appear hours or even days after exposure, making them difficult to identify without testing.

Results are reviewed with Stephanie and used to guide a personalized elimination and reintroduction approach as part of your overall wellness plan. IgG testing is used as a functional tool within a broader clinical assessment — not as a standalone diagnosis.

96-Panel

Alletess Medical Laboratory

Tests IgG reactivity to 96 common foods — a focused panel covering the foods most likely to trigger delayed immune response. A great starting point for most patients.

  • Grains, dairy & eggs
  • Meats & seafood
  • Fruits & vegetables
  • Nuts, seeds & legumes
  • Common spices & additives
184-Panel
Expanded

Alletess Medical Laboratory

Tests IgG reactivity to 184 foods — broader coverage for those with complex or difficult-to-identify sensitivities.

  • All 96-panel foods, plus
  • Expanded grain varieties
  • Additional fruits & vegetables
  • More herbs, spices & additives
  • Additional seafood & meat options

Nutritional Status

Hair Nutrient & Toxic Element Analysis

Hair tissue mineral analysis (HTMA) uses a small sample of hair to evaluate long-term nutritional status and exposure to toxic elements. Unlike blood tests that capture a single moment in time, hair analysis reflects mineral levels and toxic metal accumulation over the past 2–3 months — providing a broader picture of what your body has been exposed to and storing.

At NPS Wellness, hair nutrient analysis is particularly useful for evaluating contributors to hair loss or thinning, chronic fatigue, cognitive concerns, or as part of a comprehensive root-cause wellness evaluation.

Small hair sample — non-invasive collection
Reflects 2–3 month accumulation window
Screens both nutrient and toxic elements
Results reviewed with Stephanie
Nutrient Elements Evaluated

Essential minerals supporting cellular energy, hormones, immune function, bone health, and neurological function:

  • Calcium, Magnesium, Potassium
  • Zinc, Copper, Selenium
  • Iron, Manganese, Chromium
  • Phosphorus, Sodium, Sulfur
  • Boron, Molybdenum, Vanadium
Toxic Elements Screened

Heavy metals and toxic elements that accumulate in tissues over time:

  • Lead, Mercury, Arsenic
  • Cadmium, Aluminum, Antimony
  • Barium, Bismuth, Cesium
  • Nickel, Tin, Titanium
  • Uranium, Thallium, Tungsten

Hormone Health · Saliva Testing

Saliva LCMS Hormones 5 with 4-Point Cortisol

ZRT Laboratory — This saliva-based hormone panel uses Liquid Chromatography–Mass Spectrometry (LCMS) to measure five key sex hormones with high precision, alongside a 4-point cortisol curve that captures your cortisol pattern across the day — morning, noon, evening, and night.

Saliva testing offers a direct measure of the free, bioavailable fraction of hormones — the portion that is actually active in your tissues. This makes it especially useful for assessing how hormones are functioning at the cellular level, not just what’s circulating in the blood.

Saliva samples collected at home
LCMS technology — highly accurate, low detection limits
4-point cortisol collected across one day
Results reviewed with Stephanie, NP-C
Sex Hormones (LCMS)

Estradiol (E2), Estriol (E3), Progesterone (Pg), Testosterone (T), and DHEA-S — the five key hormones measured via mass spectrometry for precise free-fraction results.

4-Point Cortisol Curve

Morning, noon, evening, and night cortisol samples reveal your full diurnal rhythm — showing whether your adrenal response is appropriately timed and balanced throughout the day.

Why Saliva?

Unlike blood serum, saliva reflects free (unbound) hormone levels — the bioavailable portion that can enter cells and exert effects. This makes it particularly relevant for evaluating symptoms when blood levels appear “normal.”

Best Suited For

Patients experiencing fatigue, mood changes, low libido, PMS, perimenopause or menopause symptoms, sleep disruption, anxiety, or irregular energy patterns — especially when standard blood panels have not provided a clear picture.

Understand What’s Really Going On

Advanced functional testing is available as part of your Root-Cause Wellness Consult with Stephanie, NP-C. Book a consultation to discuss which tests are right for your goals.

Stephanie Kertes, NP-C — Responsible Practitioner & Oversight Provider

Licensed in the State of Colorado  ·  Florida (Telehealth Only)  ·  Indiana  ·  Michigan

Medical services at this facility are overseen by a licensed healthcare provider with prescriptive authority who serves as the Responsible Practitioner. The Responsible Practitioner establishes clinical protocols, supervises medical services, and ensures patient safety and regulatory compliance.

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